HPLC法同时测定黄酒中4种非法食品添加剂
陈军
常州市粮油质量监督检测站,江苏常州 213000
Simultaneous Determination of Four Kinds of Illegal Food Additives in Chinese Rice Wine by HPLC
陈军
Supervision and Examination Station of Grain and Oil Products Quality of Changzhou City, Changzhou 213000, China
摘要:建立高效液相色谱同时测定黄酒中安赛蜜、苯甲酸、山梨酸和糖精钠4种非法食品添加剂的方法。黄酒中的待测物质经提取后,采用C18柱分离,以甲醇-乙酸铵溶液为流动相进行洗脱,在波长230 nm处用高效液相色谱-二极管阵列检测器进行测定。安赛蜜、苯甲酸、山梨酸和糖精钠的质量浓度在0.5~200.0 μg/mL范围内与其色谱峰面积的线性关系良好(r>0.999 7),检出限为0.29~0.74 μg/L。在0.5,1.0,2.5,5.0,7.5 mg添加水平时的平均回收率在95.6%~104.0%范围内,测定结果的相对标准偏差为0.3%~1.5%(n=6)。该方法操作简便,分离效果好,灵敏度高,结果稳定可靠,适合于黄酒中安赛蜜、苯甲酸、山梨酸和糖精钠的同时测定。
Abstract:A method of simultaneous determination of four kinds of illegal food additives in Chinese rice wine by high performance liquid chromatography(HPLC)-diode array detector was established. The sample was extracted by water, detection wavelength was 230 nm, ZORBAX Eclipse XDB-C18 was used for chromatographic separation, and methanol-ammonium acetate aqueous solution was used as mobile phase. The mass concentration has good relationship with the chromatographic peak area in the range of 0.5-200.0 μg/mL(r>0.999 7), and the detection limit was 0.29-0.74 μg/L. The relative standard deviations of determination results were 0.3%-1.5%(n=6). The average recovery rates were 95.6%-104.0%. This method is simple, accurate, sensitive and suitable for the simultaneous determination of illegal food additives in Chinese rice wine.
关键词:黄酒;高效液相色谱法;安赛蜜;苯甲酸;山梨酸;糖精钠
Keywords:Chinese rice wine; HPLC; acesulfame-K; benzoic acid; sorbic acid; sodium saccharin
本文引用格式:
陈军.HPLC法同时测定黄酒中4种非法食品添加剂[J].化学分析计量,2014,23(3):61-63.
CHEN J.Simultaneous Determination of Four Kinds of Illegal Food Additives in Chinese Rice Wine by HPLC[J]. Chemical analysis and meterage,2014,23(3):61-63.
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