分析泽泻提取物中三萜类化合物
邓岳1,刘阿娜1,王小明1,周洪雷1,李运伦2,蒋海强3
1.山东中医药大学,济南 250355; 2.山东中医药大学附属医院高血压病中医临床研究中心,济南 250011; 3.山东中医药大学实验中心,济南 250355
Analysis of the Triterpenes in the Extract of Alisma Orientalis (Sam.) Juzep by HPLC-TOF-MS
邓岳1,刘阿娜1,王小明1,周洪雷1,李运伦2,蒋海强3
1. Pharmacy College of Shandong University of Traditional Chinese Medicine, Jinan 250355, China; 2. Traditional Chinese Medicine Clinical Research Base for Hypertension of Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250011, China; 3. Experimental Center of Shandong University of Traditional Chinese Medicine, Jinan 250355, China
摘要:分析和鉴定泽泻提取物中的化学成分。釆用高效液相色谱与飞行时间质谱联用技术 (HPLC-TOF-MS), 在正离子检测模式下用全扫描方式获得高分辨质谱数据,结合 Agilent Mass Hunter Qualitative Analysis 软件、PCDL、 数据库和参考文献,总结已知化学成分的质谱裂解规律,对泽泻中的三萜类成分进行定性鉴别。泽泻提取物中共鉴 定了 22 种三萜化合物。实验结果为泽泻的物质基础及质量控制研究提供了理论依据。
Abstract:The chemical constituents of the extract of Alisma orientalis (Sam.) Juzep were analyzed and authenticated. HPLC-TOF-MS technology was applied for high resolution mass spectrometry data operated in positive ion mode in full scan mode. With Agilent Mass Hunter Qualitative Analysis software, PCDL database and references, separating components in Alisma orientalis (Sam.)Juzep, based on the summary of fragmentation regularities of known chemical composition, 22 triterpene compounds were identified. The experimental results further enrich the research content of the chemical composition and provide certain theoretical basis for material basis and quality control of Alisma orientalis (Sam.)Juzep.
关键词:泽泻;三萜类;化学成分;高效液相色谱与飞行时间质谱联用技术
Keywords:Alisma orientalis (Sam.) Juzep; triterpenes; chemical constituent; HPLC-TOF/MS
基金:国家自然科学基金资助课题
本文引用格式:
邓岳,刘阿娜,王小明,等.HPLC-TOF-MS分析泽泻提取物中三萜类化合物[J].化学分析计量,2015,24(6):11-14 .
DENG Y,LIU A N,WANG X M,et al.Analysis of the Triterpenes in the Extract of Alisma Orientalis (Sam.) Juzep by HPLC-TOF-MS[J]. Chemical analysis and meterage,2015,24(6):11-14.
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