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《化学分析计量》 2016年第05期
DOI:10.3969/j.issn.1008-6145.2016.05.005
基于水溶性浸出物及HPLC指纹图谱评价不同贮存年限半夏的品质
王朋展1,巩丽丽2,容蓉1,姜立国3
1.山东中医药大学药学院,济南 250355; 2.山东中医药大学实验中心,济南 250355; 3.山东健坤药业有限公司,济南 250106 
Quality Evaluation of Pinellia Ternata of Different Reserve Time Based on Water-Soluble Extract and HPLC Fingerprint
王朋展1,巩丽丽2,容蓉1,姜立国3
1. School of Pharmaceutical Sciences, Shandong University of Traditional Chinese Medicine, Jinan 250355, China; 2. Experience Center of Shandong University of Traditional Chinese Medicine, Jinan 250355, China; 3. Shandong Jiankun Pharmaceutical Co., Ltd., Jinan 250106, China 
摘要:研究不同贮存年限半夏药材的浸出物,建立浸出物的 HPLC 特征指纹图谱,为半夏药材品质评控提供参 考。浸出物测定方法采用药典法;HPLC 指纹图谱的色谱条件:采用 C18 色谱柱 (150 mm ×4.6 mm,5 μm),以水 -甲醇为流动相,梯度洗脱,流量为 0.8 mL/min,检测波长为 260 nm,柱温为 25℃,进样体积为 50 μL。采用相似度评 价及聚类分析技术揭示 14 批样品的相似性及差异性。14 批半夏浸出物有 12 批合格,2 批不合格。建立 14 批半夏 浸出物样品的高效液相指纹图谱,确定了 3 个共有峰,共有峰保留时间的相对标准偏差小于 2%,峰面积的相对标准 偏差差异较大。1#~7#半夏样品有 12 个共有峰,共有峰保留时间的相对标准偏差小于 1.5%,峰面积的相对标准偏差 差异较大。各批次药材化学成分组成及含量均存在一定差异。以半夏浸出物数据与其高效液相色谱指纹图谱数据 为基础,将指纹图谱相似度评价与聚类分析结合起来,用浸出物含量及评价软件测评结果对半夏品质进行综合评估, 可以更精确地对半夏药材进行质量控制。 
Abstract:HPLC fingerprint of Pinellia Ternata was established which were derived from different harvest time and provided reliable method and scientific basis for their quality control. The contents of water-soluble extract was determined by Chinese Pharmacopoeia. The HPLC fingerprint chromatographic separation was achieved on a C18 column (150 mm×4.6 mm, 5 μm), water and methanol solution was used as mobile phase in gradient elution program with the flow rate of 0.8 mL/min, the column temperature of 25℃, the injection volume of 50 μL and the detection wavelength of 260 nm. The similarity evaluation and clustering analysis were used to reveal similarities and differences in 14 batches of samples. The extract of 12 batches in 14 batches Pinelliae Rhizoma were qualified and 2 batches failed. The HPLC fingerprint of 14 batches of Pinelliae Rhizoma extract were set up and 3 common peaks were obtained, the RSD of the relative retention time of the common peaks was below 2%, the RSD of the relative peak area was difference. 1#-7# samples had 12 common peaks, the RSD of the relative retention time of the common peaks was below 1.5%, the RSD of the relative peak area was difference. 14 batches of medicinal in chemical composition and content were difference. Based on water-soluble extract data and HPLC fingerprint data and combined the fingerprint similarity evaluation system and cluster analysis, this comprehensive evaluation can be more precise for the Pinelliae Rhizoma quality control. 
关键词:半夏;浸出物;高效液相色谱指纹图谱;聚类分析;相似度分析 
Keywords:Pinelliae Rhizoma; water-soluble extract; HPLC fingerprint; clustering analysis; similarity analysis 
基金:科技部科技重大专项 
本文引用格式:
王朋展,巩丽丽2,容蓉,等.基于水溶性浸出物及HPLC指纹图谱评价不同贮存年限半夏的品质[J].化学分析计量,2016,25(5):19-23. 

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