流动注射法测定鲜木薯中氰化物
韦小烨,黄一帆,陈桂鸾,班雁华,陆云
广西分析测试研究中心,南宁 530022
Determination of cyanide in fresh cassava by flow injection method
韦小烨,黄一帆,陈桂鸾,班雁华,陆云
Guangxi Center for Analytical Testing and Research, Nanning 530022, China
摘要:建立稀氢氧化钠溶液超声提取鲜木薯中的氰化物,再用流动注射分析仪测定其含量的方法。将粉碎的样品以2 g/L氢氧化钠溶液提取,取上清液用流动注射分析仪进行定量分析。氰化物质量浓度在0~0.500 μg/mL范围内与吸光度呈良好线性关系,线性方程为y=9.147×10-6x-6.454×10-2,相关系数为1.0000 ,方法检出限为0.05 mg/kg,高、中、低3个浓度样品氰化物检测结果的相对标准偏差为1.6%~3.6%(n=6),加标回收率为96.8%~97.8%。采用该方法对木薯样品进行测定,测定结果满足国标GB 5009.36-2016食品中氰化物的检测误差要求。该方法线性范围宽,重复性好,可用于鲜木薯中氰化物含量的快速检测。
Abstract:A method for determining cyanide in fresh cassava was set up with ultrasonic extraction of dilute sodium hydroxide solution and determination by flow injection analyzer. After the pulverized samples being extracted with 2 g/L
sodium hydroxide solution, the supernatant was measured by a flow injection analyzer. The mass concentration of cyanide had a good linear relationship with the absorbance in the range of 0-0.500 μg/mL. The linear equation was y=9.147×10-6x- 6.454×10-2 with the correlation coefficent of 1.0000. The method detection limit was 0.05 mg/kg. The relative standard deviations of the detection results of cyanide with three level concentration were 1.6%-3.6%(n=6), and the recovery rates of the added standard were 96.8%-97.8%. This method was used to test the samples of cassava, and the results met the requirements of the determination of cyanide in food in the national standard GB 5009.36-2016. This method has a wide linear range and good repeatability, and it can be used for rapid detection of cyanide content in fresh cassava.
关键词:流动注射法;氰化物;超声提取;木薯
Keywords:flow injection method; cyanide; ultrasonic extraction; cassava
基金:广西科技计划项目(2016ACZ06;2015ACZ06)
本文引用格式:
韦小烨,黄一帆,陈桂鸾,等.流动注射法测定鲜木薯中氰化物[J].化学分析计量,2018,27(3):20-23.
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