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《化学分析计量》 2019年第02期
DOI:10.3969/j.issn.1008-6145.2019.02.021
高效液相色谱法测定食用植物油中6 种合成抗氧化剂
韩焕美,闫荣军,张爱霞,郑新华,陈晞,何桂华
济南出入境检验检疫局,济南 250014 
Determination of 6 synthetic antioxidants in edible vegetable oil by high performance liquid chromatography
韩焕美,闫荣军,张爱霞,郑新华,陈晞,何桂华
Jinan Bureau of Entry–Exit Inspection and Quarantine, Jinan 250014, China 
摘要: 建立高效液相色谱法测定食用植物油中没食子酸丙酯、叔丁基对苯二酚、叔丁基对羟基茴香醚、二丁基羟 基甲苯、没食子酸、山梨酸6 种合成类抗氧化剂的含量。样品经正己烷溶解,再用饱和正己烷的乙腈萃取,振荡,超 声25 min,离心后取乙腈层,定容,过0.45 μm 滤膜。以0.4% 乙酸水溶液-乙腈为流动相,流量为0.8 mL/min,梯 度洗脱,检测波长为280 nm,采用高效液相色谱法进行检测。6 种合成抗氧化剂的质量浓度在0.1~10 μg/mL 范围 内与色谱峰面积呈良好的线性关系,相关系数均大于0.999,方法检出限均为1.0~2.5 mg/kg。样品的加标回收率为 79.6%~108.9%,测定结果的相对标准偏差为2.03%~5.77%(n=6)。该方法操作简单,能同时检测6 种合成抗氧化剂, 适用于多种食用植物油中合成抗氧化剂的测定。  
Abstract:A method for the determination of propyl gallate(PG), tertiary butylhydroquinone(TBHQ), butylated hyd-roxylanisole(BHA), butylated hyd-roxytoluene(BHT), gallic acid and sorbic acid in edible vegetable oil by high performance liquid chromatography was established. The samples were dissolved in n-hexane, then extracted with saturated n-hexane acetonitrile, oscillated, ultrasound for 25 minutes, centrifuged, acetonitrile layer was taken, through 0.45 μm filter membrane after constant volume. The mobile phase was 0.4% acetic acid aqueous solution - acetonitrile, flow rate was 0.8 mL/min, gradient elution, detection wavelength was 280 nm, and the detection was carried out by high performance liquid chromatography. The mass concentration of six synthetic antioxidants in the range of 0.1-10 μg/mL showed good linear relationships with the peak area, the correlation coefficients were more than 0.999, and the detection limits were 1.0-2.5 mg/kg. The recoveries of the samples were 79.6%-108.9%, and the relative standard deviations of the results were 2.03%-5.77%(n=6). The method is simple and it can simultaneously detect six synthetic antioxidants. It is suitable for the determination of synthetic antioxidants in various edible vegetable oils. 
关键词:高效液相色谱法;食用植物油;没食子酸丙酯;叔丁基对苯二酚;叔丁基对羟基茴香醚;二丁基羟基甲苯;没食子酸;山梨酸 
Keywords:high performance liquid chromatography; edible vegetable oils; PG; TBHQ; BHA; BHT; gallic acid;sorbic acid 
本文引用格式:
韩焕美,闫荣军,张爱霞,等. 高效液相色谱法测定食用植物油中6 种合成抗氧化剂[J]. 化学分析计量,2019,28(2):89-93.
HAN H M,YAN R J,ZHANG A X,et al. Determination of 6 synthetic antioxidants in edible vegetable oil by high performance liquid chromatography[J]. Chemical analysis and meterage,2019,28(2):89-93. 

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