1. 鞍山市检验检测认证中心，辽宁鞍山 114006； 2. 辽宁科技大学化学工程学院，辽宁鞍山 114051
Determination of the stability of red pigment produced by actinomycete BOS-011 by ultraviolet-visible spectrophotometry
GUAN Nawei1, WANG Yong2
1. Anshan Inspection and Testing Certification Center, Anshan 114006, China; 2. School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114051, China
件，采用紫外可见分光光度法对其稳定性进行了研究。色素样品经发酵、分离、研磨、溶解，在527 nm 波长下测定样品溶液的吸光度。该色素溶解于大部分有机溶剂，微溶于水和正丁醇；耐热性较差；在酸性介质中稳定性优于碱性介质；光照、氧气、常用防腐剂对色素的稳定性影响较小；蔗糖、Na+ 对色素有增色和保护作用；金属离子对色素的稳定性略有影响。该方法测定结果表明放线菌BOS-011 所产红色素具有较好的着色性，可作为着色剂应用于生产中。
Abstract：An actinomycete BOS-011 which can produce red pigments was purified from the soil of Changbai
Mountain. In order to clarify the condition of application and preservation of the red pigment, the stability was studied by
ultraviolet-visible spectrophotometry. After fermentation, separation, grinding and dissolution, the absorbance of pigment
samples was determined at 527 nm. The pigment could be easily dissolved in most of organic solvent and slightly soluble
in water and n-butyl alcohol. The pigment had poor heat resistance, and showed a better stability in an acidic medium than
under alkaline condition. Light, oxygen and common preservatives had a mild effect on the stability of pigments. Sucrose
and Na+ could intense the color and protect the pigment. Metal ions had influence on the stability of the pigment. The results
of this method showed that the red pigment produced by actinomycete BOS-011 has good colouring property and could be
used as a colouring agent in production.
Keywords：microbial pigment; actinomycete; ultraviolet-visible spectrophotometry; stability
管娜巍，王勇. 紫外可见分光光度法测定放线菌BOS-011 产红色素的稳定性[J]. 化学分析计量，2019，28(3)：63-67.
GUAN N W，WANG Y. Determination of the stability of red pigment produced by actinomycete BOS-011 by ultraviolet-visible spectrophotometry[J]. Chemical analysis and meterage，2019，28(3)：63-67.