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《化学分析计量》 2019年第03期
DOI:10.3969/j.issn.1008-6145.2019.03.016
紫外可见分光光度法测定放线菌BOS-011产红色素的稳定性
管娜巍1,王勇2
1. 鞍山市检验检测认证中心,辽宁鞍山 114006; 2. 辽宁科技大学化学工程学院,辽宁鞍山 114051 
Determination of the stability of red pigment produced by actinomycete BOS-011 by ultraviolet-visible spectrophotometry
GUAN Nawei1, WANG Yong2
1. Anshan Inspection and Testing Certification Center, Anshan 114006, China; 2. School of Chemical Engineering, University of Science and Technology Liaoning, Anshan 114051, China 
摘要:自长白山土壤中纯化得到一株具有产红色素功能的放线菌BOS-011,为明确此红色素的应用和储存条 件,采用紫外可见分光光度法对其稳定性进行了研究。色素样品经发酵、分离、研磨、溶解,在527 nm 波长下测定样品溶液的吸光度。该色素溶解于大部分有机溶剂,微溶于水和正丁醇;耐热性较差;在酸性介质中稳定性优于碱性介质;光照、氧气、常用防腐剂对色素的稳定性影响较小;蔗糖、Na+ 对色素有增色和保护作用;金属离子对色素的稳定性略有影响。该方法测定结果表明放线菌BOS-011 所产红色素具有较好的着色性,可作为着色剂应用于生产中。 
Abstract:An actinomycete BOS-011 which can produce red pigments was purified from the soil of Changbai Mountain. In order to clarify the condition of application and preservation of the red pigment, the stability was studied by ultraviolet-visible spectrophotometry. After fermentation, separation, grinding and dissolution, the absorbance of pigment samples was determined at 527 nm. The pigment could be easily dissolved in most of organic solvent and slightly soluble in water and n-butyl alcohol. The pigment had poor heat resistance, and showed a better stability in an acidic medium than under alkaline condition. Light, oxygen and common preservatives had a mild effect on the stability of pigments. Sucrose and Na+ could intense the color and protect the pigment. Metal ions had influence on the stability of the pigment. The results of this method showed that the red pigment produced by actinomycete BOS-011 has good colouring property and could be used as a colouring agent in production. 
关键词:微生物色素;放线菌;紫外可见分光光度法;稳定性 
Keywords:microbial pigment; actinomycete; ultraviolet-visible spectrophotometry; stability 
本文引用格式:
管娜巍,王勇. 紫外可见分光光度法测定放线菌BOS-011 产红色素的稳定性[J]. 化学分析计量,2019,28(3):63-67.
GUAN N W,WANG Y. Determination of the stability of red pigment produced by actinomycete BOS-011 by ultraviolet-visible spectrophotometry[J]. Chemical analysis and meterage,2019,28(3):63-67. 

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