收藏我们   ENGLISH  
论文查询
范围:  
提 交
《化学分析计量》 2019年第03期
DOI:10.3969/j.issn.1008-6145.2019.03.022
高效液相色谱法测定芝麻油中的叔丁基羟基茴香醚、2,6-二叔丁基对甲酚、特丁基对苯二酚
郝媛媛1,汪瑞俊2
1. 池州市食品药品检验中心,安徽池州 247100; 2. 池州市产品质量监督检验所,安徽池州 247100 
Determination of tert-butyl hydroxyanisole, 2,6-di-tert-butyl paracresol and tert-butyl hydroquinone in sesame oil by high performance liquid chromatography
郝媛媛1,汪瑞俊2
1. Chizhou Center for Food and Drug Inspection, Chizhou 247100, China; 2. Chizhou Institute of Product Quality Supervision and Inspection, Chizhou 247100, China 
摘要:建立高效液相色谱法测定芝麻油中叔丁基羟基茴香醚(BHA)、2,6-二叔丁基对甲酚(BHT)、特丁基对 苯二酚(TBHQ) 的含量。用25 mL 甲醇分5 次涡旋超声提取芝麻油中的BHA,BHT,TBHQ,以La Chrom C18(250 mm×4.6 mm,5 μm) 为色谱柱,以甲醇-0.5% 甲酸水溶液为流动相进行梯度洗脱,流量为1.0 mL/min,进样体积为5 μL,柱温为35℃,检测波长为280 nm。BHA,BHT,TBHQ 的质量浓度在20~300 mg/L 范围内与其色谱峰面积呈良好的线性关系,相关系数大于0.999,方法检出限为0.5~1.0 mg/kg。样品加标回收率为95.1%~99.8%,测定结果的相对标准偏差为2.1%~4.7%(n=6)。该方法操作简单,试剂用量少,准确度高,精密度好,适用于芝麻油中BHA,BHT,TBHQ 含量的日常检测。 
Abstract:The HPLC method was established for the determination of tert-butyl hydroxyanisole(BHA), 2, 6-ditert- butyl paracresol(BHT) and tert-butyl hydroquinone(TBHQ) in sesame oil. BHA, BHT and TBHQ in sesame oil were extracted by 25 mL methanol with 5-time vortex ultrasound. The La Chrom C18 column (250 mm×4.6 mm, 5 μm) was used as chromatographic column, and the gradient elution with methanol-0.5% formic acid aqueous solution as tmobile phase. The flow rate was 1.0 mL/min, the injection volume was 5 μL, the column temperature was 35℃, and the detection wavelength was 280 nm. The mass concentration of BHA, BHT and TBHQ had good linear relationship with the chromatographic peak area in the range of 20-300 mg/L. The correlation coefficients were greater than 0.999, the detection limits were 0.5-1.0 mg/kg. The recoveries were 95.1%-99.8%, and the relative standard deviations of the determination results were 2.1%-4.7%(n=6). The method is simple in operation, with less reagent, high accuracy and good precision. It is suitable for routine determination of BHA, BHT and TBHQ in sesame oil. 
关键词:高效液相色谱法;芝麻油;BHA;BHT;TBHQ 
Keywords:high performance liquid chromatography; sesame oil; BHA; BHT; TBHQ 
本文引用格式:
郝媛媛,汪瑞俊. 高效液相色谱法测定芝麻油中的叔丁基羟基茴香醚、2,6- 二叔丁基对甲酚、特丁基对苯二酚[J]. 化学分析计量,2019,28(3):88-91.
HAO Y Y,WANG R J. Determination of tert-butyl hydroxyanisole,2,6-di-tert-butyl paracresol and tert-butyl hydroquinone in sesame oil by high performance liquid chromatography[J]. Chemical analysis and meterage,2019,28(3):88-91. 

全文:
相似文章:
  电喷雾检测器高效液相色谱法测定复方北豆根氨酚那敏片中灰毡毛忍冬皂苷乙和川续断皂苷乙
2024年第01期
  高效液相色谱法同时测定明胶空心胶囊中苯甲酸和山梨酸
2024年第01期
  高效液相色谱法测定化妆品原料中N-乙酰神经氨酸
2024年第01期
  高效液相色谱法测定诃子中诃黎勒酸和诃子酸
2024年第01期
  多功能针式过滤器净化超高效液相色谱法测定大米和花生中玉米赤霉烯酮
2024年第01期
  柱前衍生-高效液相色谱法测定脑蛋白水解物中多肽含量
2023年第11期
  高效液相色谱法测定化妆品中5种水杨酸酯紫外吸收剂
2023年第11期
  高效液相色谱法测定喘舒片中的硫及其结果的聚类分析
2023年第11期
  限进介质色谱柱高效液相色谱法测定动物源性食品中对乙酰氨基酚残留量
2023年第11期
  高效液相色谱法测定冷面、米线中乙二胺四乙酸二钠
2023年第10期
《化学分析计量》杂志社 版权所有,未经授权禁止复制或者建立镜像
地址:山东省济南市天桥区田庄东路3号 邮编:250031
电话:0531-85878132 85878224 85878278
鲁ICP备05018524号-6