高效液相色谱法测定芝麻油中的叔丁基羟基茴香醚、2,6-二叔丁基对甲酚、特丁基对苯二酚
郝媛媛1,汪瑞俊2
1. 池州市食品药品检验中心,安徽池州 247100; 2. 池州市产品质量监督检验所,安徽池州 247100
Determination of tert-butyl hydroxyanisole, 2,6-di-tert-butyl paracresol and tert-butyl hydroquinone in sesame oil by high performance liquid chromatography
郝媛媛1,汪瑞俊2
1. Chizhou Center for Food and Drug Inspection, Chizhou 247100, China; 2. Chizhou Institute of Product Quality Supervision and Inspection, Chizhou 247100, China
摘要:建立高效液相色谱法测定芝麻油中叔丁基羟基茴香醚(BHA)、2,6-二叔丁基对甲酚(BHT)、特丁基对
苯二酚(TBHQ) 的含量。用25 mL 甲醇分5 次涡旋超声提取芝麻油中的BHA,BHT,TBHQ,以La Chrom C18(250 mm×4.6 mm,5 μm) 为色谱柱,以甲醇-0.5% 甲酸水溶液为流动相进行梯度洗脱,流量为1.0 mL/min,进样体积为5 μL,柱温为35℃,检测波长为280 nm。BHA,BHT,TBHQ 的质量浓度在20~300 mg/L 范围内与其色谱峰面积呈良好的线性关系,相关系数大于0.999,方法检出限为0.5~1.0 mg/kg。样品加标回收率为95.1%~99.8%,测定结果的相对标准偏差为2.1%~4.7%(n=6)。该方法操作简单,试剂用量少,准确度高,精密度好,适用于芝麻油中BHA,BHT,TBHQ 含量的日常检测。
Abstract:The HPLC method was established for the determination of tert-butyl hydroxyanisole(BHA), 2, 6-ditert-
butyl paracresol(BHT) and tert-butyl hydroquinone(TBHQ) in sesame oil. BHA, BHT and TBHQ in sesame oil were
extracted by 25 mL methanol with 5-time vortex ultrasound. The La Chrom C18 column (250 mm×4.6 mm, 5 μm)
was used as chromatographic column, and the gradient elution with methanol-0.5% formic acid aqueous solution as
tmobile phase. The flow rate was 1.0 mL/min, the injection volume was 5 μL, the column temperature was 35℃, and the
detection wavelength was 280 nm. The mass concentration of BHA, BHT and TBHQ had good linear relationship with the
chromatographic peak area in the range of 20-300 mg/L. The correlation coefficients were greater than 0.999, the detection
limits were 0.5-1.0 mg/kg. The recoveries were 95.1%-99.8%, and the relative standard deviations of the determination
results were 2.1%-4.7%(n=6). The method is simple in operation, with less reagent, high accuracy and good precision. It is
suitable for routine determination of BHA, BHT and TBHQ in sesame oil.
关键词:高效液相色谱法;芝麻油;BHA;BHT;TBHQ
Keywords:high performance liquid chromatography; sesame oil; BHA; BHT; TBHQ
本文引用格式:
郝媛媛,汪瑞俊. 高效液相色谱法测定芝麻油中的叔丁基羟基茴香醚、2,6- 二叔丁基对甲酚、特丁基对苯二酚[J]. 化学分析计量,2019,28(3):88-91.
HAO Y Y,WANG R J. Determination of tert-butyl hydroxyanisole,2,6-di-tert-butyl paracresol and tert-butyl hydroquinone in sesame oil by high performance liquid chromatography[J]. Chemical analysis and meterage,2019,28(3):88-91.
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