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《化学分析计量》 2022年第07期
DOI:10.3969/j.issn.1008-6145.2022.07.002
福林酚分光光度法测定蜂毒中总蛋白质
苗会娟12,胡卫国3,徐艳梅1,郝丽娟1,高燕霞1
1.河北省药品医疗器械检验研究院,石家庄 050011; 2.华北制药股份有限公司,石家庄 050200; 3.天津大学化工学院,天津 300350 
Determination of total protein in apistoxin by forint phenol spectrophotometry
MIAO Huijuan12, HU Weiguo3, XU Yanmei1, HAO Lijuan1, GAO Yanxia1
1. Hebei Provincial Institute of Drug and Medical Device Inspection, Shijiazhuang 050011, China; 2. North China Pharmaceutical Co., Ltd., Shijiazhuang 050200, China; 3. School of Chemical Engineering, Tianjin uiversity, Tianjin 300350, China 
摘要:建立福林酚分光光度法测定蜂毒中总蛋白含量的方法。参照《中国药典》2020年版通则0731蛋白质含量测定方法第二法,以水作为溶剂,在样品溶液中加入福林酚溶液4.00 mL,于25℃下反应40 min,在750 nm波长处测定吸光度,标准曲线法定量。总蛋白质的质量浓度(x)在44.4~222.0μg/mL范围内与吸光度(y)线性关系良好,线性方程为y=3.078 8x+0.025 7,相关系数为0.999 0,定量限为4.6μg/mL。总蛋白质测定结果的相对标准偏差为1.6%(n=6),样品加标回收率为96.56%~104.53%。该方法可用于蜂毒样品中总蛋白含量的测定。 
Abstract:A method for the determination of total protein content in apistoxin by forint phenol spectrophotometry was established. Referring to the second method of protein content determination in Chinese Pharmacopoeia (2020) general principle 0731, 4.0 mL folinol solution was added to the sample solution with water as solvent, react at 25℃ for 40 min, the absorbance at a wavelength of 750 nm was measured, and quantify by standard curve method. The mass concentration of total protein had a good linear relationship with the absorbance in the range of 44.4-222.0μg/mL, the linear equation was y=3.078 8x+0.025 7, the correlation coef ficient was 0.999 0, and the limit of quantitation was 4.6μg/mL. The relative standard deviation of the total protein determination results was 1.6%(n=6), and the recovery of the sample standard addition was 96.56%-104.53%. The method can be used to determine the total protein content in apistoxin samples. 
关键词:蜂毒;总蛋白质;福林酚法 
Keywords:apistoxin; total protein; forint phenol method[9] 
基金:石家庄市科学技术研究与发展计划项目(2012000483A) 
本文引用格式:
苗会娟,胡卫国,徐艳梅,等.福林酚分光光度法测定蜂毒中总蛋白质[J].化学分析计量,2022,31(7):5.
MIAO Huijuan,HU Weiguo,XU Yanmei,et al. Determination of total protein in apistoxin by forint phenol spectrophotometry[J]. Chemical Analysis and Meterage,2022,31(7):5. 

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