收藏我们   ENGLISH  
论文查询
范围:  
提 交
《化学分析计量》 2022年第07期
DOI:10.3969/j.issn.1008-6145.2022.07.011
固相萃取-离子色谱法测定人尿液中钙、镁、氟、磷酸根离子
韩晓鸥,余波,刘顺鑫,曹忠波
辽宁省疾病预防控制中心,辽宁省环境与人群健康监测重点实验室,沈阳 110005 
Determination of calcium, magnesium, fluorine and phosphate ions in human urineby solid phase extraction-ion chromatography
HAN Xiaoou, YU Bo, LIU Shunxin, CAO Zhongbo
Liaoning Center for Disease Control and prevention, Liaoning Key Laboratory of environment and population health monitoring,Shenyang 110005, China 
摘要:建立固相萃取-离子色谱法测定人尿液中钙、镁两种阳离子和氟、磷酸根两种阴离子。采用C18固相萃取柱去除样品中杂质以消除干扰,以CS12 A型阳离子交换柱为分离柱,以甲基磺酸溶液为淋洗液,等度洗脱,测定钙、镁离子;以AS15型阴离子交换柱为分离柱,以KOH溶液为淋洗液,梯度洗脱,测定氟、磷酸根离子。钙、镁离子的质量浓度在0.25~10 mg/L范围内,氟、磷酸根离子的质量浓度分别在0.002 5~0.05 mg/L、1.25~50 mg/L范围内与色谱峰面积具有良好的线性关系,相关系数均大于0.999 0,方法检出限为0.002~0.2 mg/L。样品加标回收率为86.0%~99.5%,测定结果的相对标准偏差为0.12%~5.50%(n=6)。该方法操作简便,灵敏度高。 
Abstract:A solid phase extraction-ion chromatography was established for the determination of calcium, magnesium cations, fluorine and phosphate anions in human urine. C18 solid phase extraction column was used to remove impurities in the sample to eliminate interference. CS12A cation exchange column was used as the separation column and methyl sulfonic acid solution was used as the eluent. Calcium and magnesium ions were determined by equal elution. Fluoride and phosphate ions were determined by gradient elution with AS15 anion exchange column as separation column and KOH solution as eluent. The mass concentrations of calcium and magnesium ions in the range of 0.25-10 mg/L, fluorine and phosphate ions in the range of 0.002 5-0.05 mg/L and 1.25-50 mg/L respectively had a good linear relationship with the chromatographic peak area, the correlation coef ficients were all more than 0.999 0, and the detection limit of the method was 0.002-0.2 mg/L. The recoveries of spiked samples were 86.0%-99.5%, and the relative standard deviations of the determination results were 0.12%-5.50%(n=6). The method is simple and sensitive. 
关键词:固相萃取;离子色谱法;尿;钙离子;镁离子;氟离子;磷酸根离子 
Keywords:solid phase extraction; ion chromatography; urine; calcium ion; magnesium ion; fluorine ion; phosphateion 
本文引用格式:
韩晓鸥,余波,刘顺鑫,等.固相萃取-离子色谱法测定人尿液中钙、镁、氟、磷酸根离子[J].化学分析计量,2022,31(7):48.
HAN Xiaoou,YU Bo,LIU Shunxin,et al. Determination of calcium,magnesium,fluorine and phosphate ions in human urine by solid phase extraction-ion chromatography[J]. Chemical Analysis and Meterage,2022,31(7):48. 

全文:
相似文章:
  水中氯离子溶液标准物质的研制
2022年第04期
  气相色谱-串联质谱法测定中药材中35种农药残留的样品处理方法
2022年第03期
  超声辅助提取-固相萃取净化-高效液相色谱法测定灰渣和污泥中11种双酚类及烷基酚类化合物
2022年第03期
  离子色谱法同时测定高纯试剂中痕量甲胺、二甲胺、三甲胺和6种阳离子
2022年第03期
  固相萃取-液相色谱串联质谱法测定食品中的麻黄碱、芬氟拉明、西布曲明和酚酞
2022年第02期
  抑制电导离子色谱法测定蔬菜、药品和水中对氨基苯磺酸
2022年第01期
  离子色谱法同时检测水产品加工用水中10种阴离子
2021年第11期
  高效液相色谱-串联质谱法测定果汁及乳饮料中9种焦油色素
2021年第11期
  固相萃取-高效液相色谱法快速测定烟草用爆珠中4种苏丹红
2021年第11期
  双系统离子色谱法同时检测蓝藻培养液中阴、阳离子
2021年第08期
《化学分析计量》杂志社 版权所有,未经授权禁止复制或者建立镜像
地址:山东省济南市天桥区田庄东路3号 邮编:250031
电话:0531-85878132 85878224 85878278
鲁ICP备05018524号-6