1.核工业二三〇研究所，长沙 410007； 2.湖南省生态环境事务中心，长沙 410014
Determination of sulfur in plant by airtight digestion-inductively coupled plasma optical emission spectrometry
ZHANG Hao1, LI Yuan1, WU Liang1, ZHAO Fangzhou1, LIU Jian2, YI Wenjie2, CHEN Jie1, GUO Ziqiang1
1. Research Institute No. 230, CNNC, Changsha 410007, China; 2. Hunan Ecolongical and Environmental Affairs Center, Changsha 410014, China
摘要：建立密闭消解-电感耦合等离子体发射光谱法测定植物中硫含量的方法。以硝酸和过氧化氢为消解溶剂，利用聚丙烯离心管密闭消解样品，选择分析谱线为182.03 nm，用电感耦合等离子体发射光谱法测定植物中的硫含量。硫元素的质量浓度在0～50.0μg/mL范围内与光谱强度具有良好的线性关系，相关系数为0.999 9，方法检出限为0.000 6%。采用所建方法对湖南大米标准物质(GBW 10045a)和菠菜、绿茶样品进行6次平行测定，测定结果的相对标准偏差分别为2.23%、1.75%、1.11%；对圆白菜(GBW 10014a)、菠菜(GBW 10015a)、绿茶(GBW 10052a)、大葱(GBW 10049) 4种标准物质进行测定，测定值均在标准值不确定度范围内，相对误差分别为0.65%、0.08%、1.88%、1.12%。该方法操作简便、快捷，适用于大批量植物样品中硫的测定。
Abstract：A method for the determination of sulfur in plants by airtight digestion-inductively coupled plasma optical emission spectrometry was established. The sample was digested with nitric acid and hydrogen peroxide in a closed polypropylene centrifuge tube. The analytical line was 182.03 nm. The sulfur content in plants was determined by inductively coupled plasma optical emission spectrometry. The mass concentration of sulfur had a good linear relationship with the spectral intensity in the range of 0-50.0μg/mL, the correlation coefficient was 0.999 9, and the detection limit of the method was 0.000 6%. Hunan rice reference material (GBW 10045a), spinach and green tea samples were determined by the established method for 6 times, the relative standard deviations of the determination results were 2.23%, 1.75% and 1.11%, respectively. The four reference materials of chinese cabbage (GBW 10014a), spinach (GBW 10015a), green tea (GBW 10052a) and scallion (GBW 10049) were determined, the measured values were within the uncertainty range of the standard values, and the relative errors were 0.65%, 0.08%, 1.88% and 1.12%, respectively. The method is simple, rapid, which suitable for the determination of sulfur in a large number of plant samples.
Keywords：inductively coupled plasma optical emission spectrometry; plant; sulfur; airtight digestion
ZHANG Hao，LI Yuan，WU Liang，et al. Determination of sulfur in plant by airtight digestion-inductively coupled plasma optical emission spectrometry[J]. Chemical Analysis and Meterage，2022，31(7)：53.