化学电离-气相色谱-串联质谱法测定卷烟侧流烟气中的4种烟草特有亚硝胺
陈义坤1,崔华鹏2,陈黎2,黄龙1,刘克建2,郭军伟2,刘瑞红2,陈满堂2,罗诚浩1,刘绍锋2
1.湖北中烟工业有限责任公司技术中心,武汉 430040; 2.中国烟草总公司郑州烟草研究院,郑州 450001
Detection of 4 kinds of tobacco-specific nitrosamines in sidestream cigarette smoke by chemical ionization-gas chromatography-tandem mass spectrometry
CHEN Yikun1, CUI Huapeng2, CHEN Li2, HUANG Long1, LIU Kejian2, GUO Junwei2, LIU Ruihong2, CHEN Mantang2, LUO Chenghao1, LIU Shaofeng2
2 1 2CHEN Mantang, LUO Chenghao, LIU Shaofeng(1. Technology Center of China Tobacco Hubei Industrial Co., Ltd., Wuhan 430040, China; 2. Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, China)
摘要:建立了化学电离-气相色谱-串联质谱法测定卷烟侧流烟气中4种烟草特有亚硝胺(TSNAs)的方法。采用吸烟机对卷烟进行抽吸,利用鱼尾罩和剑桥滤片对卷烟侧流烟气进行捕集,用稀盐酸溶液进行鱼尾罩洗涤和剑桥滤片萃取,萃取液经阳离子交换固相萃取柱净化,采用氨水甲醇溶液洗脱,洗脱液经氮气吹干后用二氯甲烷定容至1 mL,过滤膜后检测,利用色谱峰面积内标法定量。4种TSNAs的质量浓度在1~500 ng/mL范围内与各目标物和内标的色谱峰面积比线性关系良好,相关系数均大于0.999 1,检出限为0.16~0.35 ng/mL。加标回收率为80.8%~107.4%,检测结果的相对标准偏差为3.2%~6.8%(n=6)。
Abstract:A method was established for determining 4 kinds of tobacco-specific nitrosamines (TSNAs) in sidestream cigarette smoke by chemical ionization-gas chromatography-tandem mass spectrometry. Cigarette was smoked by a smoking machine, the generated sidestream cigarette smoke was trapped by a fishtail chimney and cambridge filter. Dilute hydrochloric acid solution was used for washing the fishtail chimney and extracting the cambridge filter. The extracted solution was purified by a cation exchange solid phase extraction column. The extraction column was eluted with ammonia and methanol solution. The eluent was blown to dry by nitrogen gas, and then was fixed to 1 mL by using dichloromethane. After being filtered by a filter membrane, the sample was detected by gas chromatography-mass spectrometer, and quanti fied by internal standard method. The mass concentration of 4 kinds of tobacco-speci fic nitrosamines had a good linear relationship with the chromatographic peak ratio of TSNAs to internal standard compounds in the range of 1-500 ng/mL, the correlation coefficients were all more than 0.999 1. The detection limits were 0.16-0.35 ng/mL, the recoveries were 80.8%-107.4%, and the relative standard deviations of determination results were 3.2%-6.8%(n=6).
关键词:化学电离;气相色谱-串联质谱法;烟草特有亚硝胺
Keywords:chemical ionization; gas chromatography-tandem mass spectrometry; tobacco-specific nitrosamines[1]
基金:国家自然科学基金项目(21705164)
本文引用格式:
陈义坤,崔华鹏,陈黎,等.化学电离-气相色谱-串联质谱法测定卷烟侧流烟气中的4种烟草特有亚硝胺[J].化学分析计量,2022,
31(11):6.CHEN Yikun,CUI Huapeng,CHEN Li,et al. Detection of 4 kinds of tobacco-specific nitrosamines in sidestream cigarette smoke bychemical ionization-gas chromatography-tandem mass spectrometry[J]. Chemical Analysis and Meterage,2022,31(11):6.
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